Skip to main content
Figure 3 | BMC Molecular Biology

Figure 3

From: Extensive ssDNA end formation at DNA double-strand breaks in non-homologous end-joining deficient cells during the S phase

Figure 3

ssDNA (BrdU foci) in NHEJ deficient cells. M059J (NHEJ deficient) and M059K (NHEJ proficient) cells were fixed 4 h after irradiation. (A) BrdU corresponding to ssDNA was detected with immunofluorescence, and (B) cells were scored as belonging to one of the following categories: I. Weak staining in the whole nucleus; II. Increased staining in the nucleus compared to I, dots are present in the whole nucleus; III. Strong staining in the nucleus, many dots with high intensity in the whole nucleus. At least 100 cells in three experiments were scored. Error bars represent the SD.

Back to article page

BMC Molecular Biology

ISSN: 1471-2199

Contact us