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Figure 8 | BMC Molecular Biology

Figure 8

From: Regulation of poly(ADP-ribose) polymerase-1 (PARP-1) gene expression through the post-translational modification of Sp1: a nuclear target protein of PARP-1

Figure 8

rPARP-1 promoter activity in PARP-1+/+ and PARP-1-/- cells. (A) The recombinant plasmids PCR3 and PCR3F2/F3/F4m were transfected into both PARP-1+/+ and PARP-1-/- cells grown with or without the PARP-1 inhibitor PJ34. CAT activities were measured and normalized to the amount of hGH secreted into the culture medium. Values are expressed as ((%CAT activity/100 μg proteins)/ng hGH). Asterisks (*) indicate CAT activities from cells exposed to PJ34 that are statistically different from those measured when cells are transfected with pCR3 in the absence of inhibitor whereas † corresponds to CAT activities in PARP-1-/- cells that are statistically different from those measured in PARP-1+/+ cells (P < 0.005; paired samples, t-test). S.D. is also provided. (B) The plasmids PCR3, -92α5CAT and α6–84 were transfected into both PARP-1+/+ and PARP-1-/- cells and CAT activity (expressed as the ratio of CAT activity from PARP-1-/- cells over that measured in PARP-1+/+ cells (considered as 100%)) measured and normalized as detailed above. Asterisks (*) correspond to CAT activities in PARP-1-/- cells that are statistically different from those measured in PARP-1+/+ cells (P < 0.005; paired samples, t-test).

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