Figure 8
Western blot analysis of recombinant FLAG-tag fusion oPRP2 protein. Conditioned media from HEK 293 cells transiently transfected with each gene were collected, and the purified proteins (1 ng) were loaded on to separate lanes. The proteins were separated by SDS-PAGE and specific proteins were detected by Western blot analysis using an anti-FLAG antibody. MW Marker: molecular weight marker.