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Figure 5 | BMC Molecular Biology

Figure 5

From: Control of gag-pol gene expression in the Candida albicans retrotransposon Tca2

Figure 5

The Tca2 long terminal repeat promoter, but not the gag-pol junction promoter, is heat-shock inducible. Either the Tca2 LTR promoter, or the newly identified gag-pol junction promoter, was cloned upstream of a promoter-less copy of the S. thermophilus lacZ gene integrated into the C. albicans genome at the ADE2 locus. β-galactosidase specific activities were measured in lysates from cultures growing at 30°C (solid bars) or after growth at 23°C followed by a two-hour heat-shock at 37°C (hatched bars). The gag-pol junction promoter activity was assayed in this way using either gag TGA variant (gag-TGA-pol) or TGT sense codon (gag-TGT-pol). The specific activity of the junction promoter constructs is indicated on the bar chart as a percentage of the activity directed by the LTR promoter (100%). Bars represent the means of three independent cultures. Error bars represent +/- 1 standard deviation.

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