Figure 2

The influence of the gag-pol junction region in directing pol expression. The Tca2 gag-pol junction region was cloned in between translationally-fused lacZ and luc genes in the dual reporter vector pAC98-U. Panel A: the graph shows the normalised level of downstream luc gene expression measured in cells transformed with the vectors indicated in the 3' deletion series (see panel B). Luciferase expression was first calculated as the ratio of luciferase to β-galactosidase levels, which was then expressed as a percentage of the same ratio measured using the parental vector (pAC98-U; control lacZ-luc). Bars represent means of independent transformants +/- 1 standard deviation (n = 3). Constructs tested all contain 5xEK repeats upstream of the gag stop codon (see Figure 1).