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Table 1 Dynamic range of template RNA amount for the five commercial RT systems tested.

From: Detection limits of several commercial reverse transcriptase enzymes: impact on the low- and high-abundance transcript levels assessed by quantitative RT-PCR

Commercial name Type of RTa Dynamic range (ng)
Omniscript ? 50–2,000
PowerScript MMLV 1,000–5,000
Sensiscript ? < 50
SuperScript II MMLV 1–5,000
SuperScript III MMLV 0.01–5,000
  1. aBoth the Omniscript and the Sensiscript RTases retain RNase H activity. They are different from the RTases of Moloney murine leukaemia virus (MMLV) or avian myeloblastosis virus (AMV) origin (Sensiscript and Omniscript reverse transcriptase handbook, Qiagen). The Sensiscript RTase is a recombinant heterodimeric enzyme expressed in E. coli. TheSuperScript II and SuperScript III RTases are an engineered version of MMLV RTase with reduced RNase H activity and increased thermal stability. The PowerScript RTase is a point mutant of MMLV RTase, which lacks the RNase H enzyme and retains wild-type polymerase activity, thus being able to synthesise longer cDNA fragments [35].