Figure 2From: Detection limits of several commercial reverse transcriptase enzymes: impact on the low- and high-abundance transcript levels assessed by quantitative RT-PCREffect of RT reaction dilution on the measurements of PCR amplifiable EGFP cDNA. Quantitative PCR was performed in triplicate on undiluted and diluted (1:50) RT samples prepared with each of the five commercial RT systems. The same quantity of EGFP mRNA (0.1 pg) was used to spike RT reactions in the presence of different amounts (0 to 2,000 ng) of background RNA.Back to article page