Figure 2

Effect of RT reaction dilution on the measurements of PCR amplifiable EGFP cDNA. Quantitative PCR was performed in triplicate on undiluted and diluted (1:50) RT samples prepared with each of the five commercial RT systems. The same quantity of EGFP mRNA (0.1 pg) was used to spike RT reactions in the presence of different amounts (0 to 2,000 ng) of background RNA.