Figure 4

Splice-regulatory motifs in the alternatively spliced ADAM15 region. Predicted alternative splicing-regulatory protein recognition motifs within and flanking the alternatively used ADAM15 exons. The exon-intron structure of the human alternatively spliced ADAM15 region is shown at the top. The exons are shown as black boxes. The exon 21b-unique 3'-region is indicated with grey. The open boxes indicate the location of the putative cryptic exons eC-19 and -20. The dashed lines indicate the possible splicings of the alternatively used exons 19–21 and solid lines indicate the constitutive splicing. For simplicity, the lines do not depict the alternative use of exon-20 5'-splice sites (differing by only three nucleotides). The alternative splicing-associated sequence motifs are indicated by the symbols explained in the key shown below the figure. The different motif classes are shown separately in the sectors indicated on the right and explained in the key. The locations of putative branch sites are indicated with the small arrows on top of the pyrimidine tracts (green bars) under the splice-regulatory motif sectors. The human-mouse sequence-conservation is visualised with mVista and PiP/BlastZ under the splice-regulatory motif panel. For easier distinction, the exonic regions are shaded in the splice-regulatory motif and sequence identity panels. Also the nucleotide numbering relative to the translation start is shown under the panels