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Table 2 The time required to lyse 50% of fibrin clot or Platelet-rich clot (T50%) and the concentration required to obtain 50% clot lysis (C50) with SAK or RGD-SAK

From: Characterization of a novel bifunctional mutant of staphylokinase with platelet-targeted thrombolysis and antiplatelet aggregation activities

Compound

Concentration (nM)

T50% of fibrin clot (min)

T50% of platelet-rich clot (min)

C50 of fibrin clot lysis (nM)

C50 of platelet-rich clot lysis (nM)

SAK

50

195 ± 8.7

198 ± 8.6

80 ± 8.5

200 ± 8.3

 

100

161 ± 9.3

166 ± 7.8

  
 

200

138 ± 8.5

123 ± 5.6

  

RGD-SAK

50

189 ± 9.9

93 ± 3.6

76 ± 8.3

38 ± 3.9

 

100

151 ± 6.3

59 ± 2.9

  
 

200

131 ± 9.7

33 ± 3.8

  
  1. The activity of RGD-SAK or SAK was comparable in fibrin clot lysis assay at various corresponding concentrations.T50% was significantly shortened for each corresponding concentration (RGD-SAK vs SAK, P < 0.01). Results shown represent means ± SD from 6 clots for each concentration of SAK or RGD-SAK. C50 of fibrin clot lysis was not significantly different between SAK and RGD-SAK, while C50 of platelet-rich clot lysis caused by SAK was much higher than that of RGD-SAK (P < 0.01).