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Figure 2 | BMC Molecular Biology

Figure 2

From: Characterization of a novel bifunctional mutant of staphylokinase with platelet-targeted thrombolysis and antiplatelet aggregation activities

Figure 2

Physicochemical and biological properties of RGD-SAK. (A) HPLC analysis of RGD-SAK. A single symmetrical peak with a purity > 98% was indicated in the HPLC analytical chart. (B) Molecular mass determination of purified RGD-SAK by MALDF-TOF mass spectrometry. Peaks corresponding to various protonated RGD-SAK species are marked. The mass analysis determined that the RGD-SAK has a molecular weight of 15.4 KDa. (C) The secondary structure contents of RGD-SAK and SAK analyzed by far-UV CD spectrum. The secondary structure revealed by the curve of the two proteins were very similar. (D) RGD-SAK fibrinolysis activity assayed on fibrin plate. The numbers above the well represent various concentration of samples. 1–5 represent various concentrations of standard SAK (6.5 IU, 12.5 IU, 25 IU, 50 IU, 100 IU/well, respectively). Duplicate wells (6–10) corresponding to 1–5 were included. 11–13 represent various diluted RGD-SAK determined by comparing with the standard SAK. Three duplicated sets (14–16, 17–19, 20–22) of RGD-SAK corresponding to 11–13 were also shown.

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BMC Molecular Biology

ISSN: 1471-2199

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