Figure 5

Overexpression and functional inactivation of C/EBPδ in primary KCs. Human KCs were transfected with mouse C/EBPδ. RNAs were extracted at the indicated time and RT-PCR is shown for the indicated p63 target genes (Left Panels). In the Right Panels, C/EBPδ inactivation was performed in human KCs with siRNA oligonucleotide 2 of Fig. 4 and with a scramble oligo, in parallel. RT-PCR analysis of the genes in Left Panels is shown. The increase of c-Jun in RNAi-treated cells can only be visualized by low cycles RT-PCR, where a signal from the control oligo RNA is not detectable. On the Right part of the Figure, the effect of RNAi inactivation of p63 is depicted, according to published data and to Ref. 19.