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Figure 2 | BMC Molecular Biology

Figure 2

From: Reciprocal regulation of p63 by C/EBP delta in human keratinocytes

Figure 2

Regulation of C/EBPδ in HaCaT and primary keratinocytes. A. Upper Panel. RT-PCR analysis of the C/EBPs in HaCaT cells before and after 3 and 72 hours in differentiation medium. cK1 was used as control for differentiation, NF-YB as internal control. Lower Panel. Western blot analysis of HaCat extracts with the indicated antibodies. B. Immunofluorescence analysis of growing and differentiated HaCaT [72 hours] using the indicated antibodies. cK1 was used to control for differentiation. Ctl refers to cells stained with the rabbit secondary antibody only. C. RT-PCR analysis as in A, except that primary human keratinocytes were used, before or after 3 days in differentiation medium. cK1 and cK14 were used as markers of differentiation. D. Same confocal experiments as in B, using human primary keratinocytes.

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