Examination of PITX3 mutant forms for dominant-negative effect. The data are presented as luciferase values observed in co-transfections of the reporter with the PITX3 plasmids relative to the activity of same reporter with empty pcDNA3.1 vector only; all experiments were performed in B3 lens epithelial cells. Data for S13N (top), G219fs (middle) and K111E mutants (bottom) are shown. The green line connects values observed in transient co-transfections of 0.5-, 1-, 1.5- and 2-μg of wild-type PITX3 and the reporter (1 μg). The blue line connects values observed in transient co-transfections of 0.5-, 1-, 1.5- and 2-μg of mutant PITX3 with the reporter (1 μg). The values observed in experiments when the reporter (1 μg) was co-transfected with 0.5 μg of wild-type PITX3 together with increasing amounts of S13N, G219fs or K111E mutants (0.5-, 1- and 1.5-μg) are joined by a red line.