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Table 2 Rates of synthesis and degradation of c-fos and c-fos-2 transcripts upon serum stimulation

From: Alternative splicing of c-fos pre-mRNA: contribution of the rates of synthesis and degradation to the copy number of each transcript isoform and detection of a truncated c-Fos immunoreactive species

 

c-fos

c-fos-2

NIH 3T3 cells

amount (molec/pg)

half-life (min)

synthesis (molec/min)

amount (molec/pg)

half-life (min)

synthesis (molec/min)

confluent

12.1

17.9

0.47

12.8

2.6

3.41

quiescent

2.6

17.5

0.10

2.2

2.3

0.66

serum (15 min)

116.1

17.1

4.71

28.1

2.3

8.47

serum (60 min)

77.1

8.5

6.29

10.2

2.7

2.62

  1. Experimental conditions are as in Figs. 4 and 6. Confluent cell data are those in Fig. 4.
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BMC Molecular Biology

ISSN: 1471-2199

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