Alternative splicing of c-fos pre-mRNA: contribution of the rates of synthesis and degradation to the copy number of each transcript isoform and detection of a truncated c-Fos immunoreactive species

Table 1 Absolute steady-state levels of transcripts encoding proteins of the Fos family in mouse lung

mouse	c-fos	c-fos-2	fosB	fra1	fra2
1	2.80	0.59	0.035	0.96	1.93
2	4.52	0.62	0.039	0.98	2.42
3	3.37	0.81	0.033	1.03	2.48
4	3.04	0.66	0.030	1.03	1.63
5	2.00	0.49	0.022	1.15	2.73
6	3.08	0.47	0.023	1.26	2.34
7	2.14	0.16	0.012	1.26	2.04
8	2.41	0.41	0.018	1.44	2.60
9	3.57	0.61	0.022	1.51	2.61
10	3.71	0.88	0.033	1.29	2.82
11	4.16	0.42	0.014	1.13	2.67
12	3.90	0.58	0.038	1.37	2.94
mean ± SEM	3.22 ± 0.23	0.56 ± 0.053	0.027 ± 0.003	1.20 ± 0.050	2.44 ± 0.11

Male BALB/c mice of 7 weeks of age were obtained from Charles River Laboratories (Spain). Following a 3-day acclimation period animals were individually killed by cervical dislocation. The lung was removed and immediately frozen in liquid nitrogen. Mice were handled according to the norms stipulated by the European Community. The investigation was performed after approval by the Ethical Committee of the University of Córdoba (Spain). Data are transcript molecules/pg of total RNA.

ISSN: 1471-2199