Time-courses of c-fos transcripts in mouse kidney, lung and liver in response to AmD. Male BALB/c mice (see legend of Table 1) were intraperitoneally injected with 2 mg kg-1 body weight of AmD dissolved in phosphate-buffered saline. Animals injected with phosphate-buffered saline served as vehicle controls. Total RNA was extracted at the indicated times. Data are the means of c-fos (triangles) or c-fos-2 (circles) molecules/pg of total RNA ± SEM (n = 3 mice). Data at 0 min represent the mean values ± SEM of 5 control mice. No time-related effect was noted in these vehicle controls. Some error bars are not visible because of small standard errors. Statistical significance was evaluated using analysis of variance followed by post hoc multiple comparison according to the Student-Newman-Keuls method. Significant differences relative to control animals are indicated by filled-in symbols. For each transcript, the maximal fold increment is given in parentheses.