Figure 2From: XRCC1 and PCNA are loading platforms with distinct kinetic properties and different capacities to respond to multiple DNA lesionsRecruitment of XRCC1 and PCNA at DNA damage sites in living cells. (A) Schematic representation of the fluorescent fusion proteins. (B) Live cell imaging of a microirradiated Hela cell coexpressing GFP-XRCC1 and RFP-PCNA. Accumulation of GFP-XRCC1 can be observed immediately after microirradiation, while RFP-PCNA accumulates with a short delay of about 2–10 s (indicated by arrows). (C) Quantitative evaluation of recruitment kinetics showing mean curves. Error bars represent the standard error of the mean. Immediate and fast recruitment of GFP-XRCC1 precedes slow and constant recruitment of RFP-PCNA at DNA damage sites. Scale bar, 5 μm.Back to article page