Table 2 Plasmids used in study

pTMP100

pMK4 containing S. aureus P _rpsJ :gfp:luxABCDE

Hill laboratory collection

pTRC99A

rrnB T1, rrnB T2

27

pDONRP4-P1R

Entry vector to clone attB4 and attB1 flanked PCR products, Km^r, Cm^r, ccdB⁺

Invitrogen

pDONR221

Entry vector to clone attB1 and attB2 flanked PCR products, Km^r, Cm^r, ccdB⁺

Invitrogen

pDONR P2R-P3

Entry vector to clone attB2 and attB3 flanked PCR products, Km^r, Cm^r, ccdB⁺

Invitrogen

pDESTR4-R3

Destination vector, contains attR4/R3 sites. Recombines with entry clones in a Multisite Gateway LR reaction, Ap^r, Cm^r, ccdB⁺

Invitrogen

pHB201

B. subtilis ori-pBR322, ori-1060, cat 86:lacZα Cm^r Em^r

15

pMK4

Gram-Positive Shuttle vector, Ap^R, Cm^r

28

pSB2018

pMK4 containing xylR P _xylA :gfp

8

pSB2026

pMK4 containing xylR P _xylA :luxABCDE

Hill laboratory collection

pUNK1

Gram-positive shuttle vector, Em^r

14

pDEST-pMK4

pMK4 containing attR4 and attR3 sites for recombination with entry clones in an LR reaction. Ap^r, Cm^r, ccdB⁺

This work

pDEST-pUNK1

pUNK1 containing attR4 and attR3 sites for recombination with entry clones in an LR reaction. Em^r, Cm^r, ccdB⁺

This work

pDEST-pHB201

pHB201 containing attR4 and attR3 sites for recombination with entry clones in an LR reaction. Em^r, Cm^r, ccdB⁺

This work

pSB3000

3 fragment expression cassette in pMK4. xylR P _xylA :gfp:rrnBT1T2 (0 attB)

This work

pSB3002

3 fragment expression cassette in pMK4. xylR P _xylA :gfp:rrnBT1T2 (2 attB)

This work

pSB3004

3 fragment expression cassette in pMK4. xylR P _xylA :gfp:rrnBT1T2 (4 attB)

This work

pSB3005

3 fragment expression cassette in MK4. P _xylA :gfp:luxABCDE rrnBT1T2 (4 attB)

This work

pSB3007

3 fragment expression cassette in pUNK1. P _xylA :gfp:luxABCDE:rrnBT1T2 (4 attB)

This work

pSB3010

3 fragment expression cassette in pMK4. xylR P _xylA :luxABCDE:rrnBT1T2 (0 attB)

This work

pSB3012

3 fragment expression cassette in pMK4. xylR P _xylA :luxABCDE:rrnBT1T2 (2 attB)

This work

pSB3014

3 fragment expression cassette in pMK4. xylR P _xylA :luxABCDE:rrnBT1T2 (4 attB)

This work

pSB3024

3 fragment expression cassette in pHB201. P _sspA gfp:luxABCDE:rrnBT1T2 (4 attB)

This work