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Table 2 Plasmids used in study

From: Construction and evaluation of multisite recombinatorial (Gateway) cloning vectors for Gram-positive bacteria

Plasmid Features of plasmid Reference/source
pTMP100 pMK4 containing S. aureus P rpsJ :gfp:luxABCDE Hill laboratory collection
pTRC99A rrnB T1, rrnB T2 27
pDONRP4-P1R Entry vector to clone attB4 and attB1 flanked PCR products, Kmr, Cmr, ccdB+ Invitrogen
pDONR221 Entry vector to clone attB1 and attB2 flanked PCR products, Kmr, Cmr, ccdB+ Invitrogen
pDONR P2R-P3 Entry vector to clone attB2 and attB3 flanked PCR products, Kmr, Cmr, ccdB+ Invitrogen
pDESTR4-R3 Destination vector, contains attR4/R3 sites. Recombines with entry clones in a Multisite Gateway LR reaction, Apr, Cmr, ccdB+ Invitrogen
pHB201 B. subtilis ori-pBR322, ori-1060, cat 86:lacZ╬▒ Cmr Emr 15
pMK4 Gram-Positive Shuttle vector, ApR, Cmr 28
pSB2018 pMK4 containing xylR P xylA :gfp 8
pSB2026 pMK4 containing xylR P xylA :luxABCDE Hill laboratory collection
pUNK1 Gram-positive shuttle vector, Emr 14
pDEST-pMK4 pMK4 containing attR4 and attR3 sites for recombination with entry clones in an LR reaction. Apr, Cmr, ccdB+ This work
pDEST-pUNK1 pUNK1 containing attR4 and attR3 sites for recombination with entry clones in an LR reaction. Emr, Cmr, ccdB+ This work
pDEST-pHB201 pHB201 containing attR4 and attR3 sites for recombination with entry clones in an LR reaction. Emr, Cmr, ccdB+ This work
pSB3000 3 fragment expression cassette in pMK4. xylR P xylA :gfp:rrnBT1T2 (0 attB) This work
pSB3002 3 fragment expression cassette in pMK4. xylR P xylA :gfp:rrnBT1T2 (2 attB) This work
pSB3004 3 fragment expression cassette in pMK4. xylR P xylA :gfp:rrnBT1T2 (4 attB) This work
pSB3005 3 fragment expression cassette in MK4. P xylA :gfp:luxABCDE rrnBT1T2 (4 attB) This work
pSB3007 3 fragment expression cassette in pUNK1. P xylA :gfp:luxABCDE:rrnBT1T2 (4 attB) This work
pSB3010 3 fragment expression cassette in pMK4. xylR P xylA :luxABCDE:rrnBT1T2 (0 attB) This work
pSB3012 3 fragment expression cassette in pMK4. xylR P xylA :luxABCDE:rrnBT1T2 (2 attB) This work
pSB3014 3 fragment expression cassette in pMK4. xylR P xylA :luxABCDE:rrnBT1T2 (4 attB) This work
pSB3024 3 fragment expression cassette in pHB201. P sspA gfp:luxABCDE:rrnBT1T2 (4 attB) This work