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Table 1 Primers for PCR and sequencing

From: Construction and evaluation of multisite recombinatorial (Gateway) cloning vectors for Gram-positive bacteria

Gateway primers

Sequence (5-3')

Use

Term attB2F

GGGGACAGCTTTCTTGTACAAAGTGGAAATCAGAACGCAGAAGCGGTC

Amplification of terminator

Term attB3R

GGGGACAACTTTGTATAATAAGTTGTTTAAACTGGCAGTTTATGGCGGGC

Amplification of terminator

Lux E attB2R

GGGGACCACTTTGTACAAGAAAGCTGGGTTCAACTATCAAACGCTTCGG

Amplification of lux reporter

GFP attB2R

GGGGACCACTTTGTACAAGAAAGCTGGGTGACACATTTATTTGTATAGTTC

Amplification of gfp reporter

GFPopt attB1F

GGGGACAAGTTTGTACAAAAAAGCAGGCTAGGAGGAATAAAAAATGAGTAAAGGCGAAGAAC

Amplification of gfp reporter

XylRA attB4F

GGGGACAACTTTGTATAGAAAAGTTGCGTTGACTTAACTAACTTATAG

Amplification of xylR P xylA

XylRA attB1R

GGGGACTGCTTTTTTGTACAAACTTGTTGA TTTAAGTGAACAAGTTTATC

Amplification of xylR P xylA

XylA attB4F

GGGGACAACTTTGTATAGAAAAGTTGAAACATTGAAATAAACATTTATTTTGTATATGATG

Amplification of P xylA

XylA attB1R

GGGGACTGCTTTTTTGTACAAACTTGTTGATTTAAGTGAACAAGTTTATC

Amplification of P xylA

LuxA attB1F

GGGGACAAGTTTGTACAAAAAAGCAGGCTAGGAGGACTCTCTATGAAATTTGGAAAC

Amplification of lux reporter

SASP attB4F

GGG GAC AAC TTT GTA TAG AAA AGT TGT TTA AAC GCT GGA ACC TTT TGT TCC CAA AAG

Amplification of P sspA

SASP attB1R

GGG GAC TGC TTT TTT GTA CAA ACT TGT TTT ATT TAG TAT GGT TGG GTT AAC TGG

Amplification of P sspA

attR3

GGGGCGTTTAAACAAGCTTGTAAAACGACGGCCAGTG

sequencing

PMEattR4

GGGTTTAAACAACTTTGTATAGAAAAGTTGAACGAG

sequencing

Ccdb Rev

AGGAAGGGATGGCTGAGGTC

sequencing

Cm R4-R3F

CAGGCGGGCAAGAATGTGA

sequencing