Skip to main content
Figure 7 | BMC Molecular Biology

Figure 7

From: Construction and evaluation of multisite recombinatorial (Gateway) cloning vectors for Gram-positive bacteria

Figure 7

Comparison of vectors in L. monocytogenes. L. monocytogenes 7973 plasmids based on the shuttle vector pMK4 (, ▲; pSB3005) or pUNK1 (□ ■; pSB3007) containing the gfp:luxABCDE operon under the control of the P xylA promoter (see Table 2). Cells were grown in MWB at 37°C and luminescence (closed symbols, Relative Light Units; RLU)) and growth measurements (open symbols) were taken at intervals. Reporter gene data are presented as RLU/OD600 nm to account for increasing cell number during the experiment.

Back to article page