Figure 6

Reporter gene repression kinetics. S. aureus RN4220 containing reporter plasmids pSB3004 (□, ■; 4 att sites), pSB3002 (△, ▲; 2 att sites) and pSB3000 (○, ●; no att sites) were grown to mid-log phase in Tris Minimal Succinate medium with 5 μg ml^-1 Cm and 0.5% xylose. Bacteria were washed and resuspended in an equal volume of medium containing 5 μg ml^-1 Cm and 1% glucose. Aliquots were placed in a 96 well microtitre plate and incubated at 37°C in a Tecan Genios Pro. Panel A: fluorescence (solid symbols, (Relative Fluorescence Units; RFU) and absorbance (open symbols) were measured at 10 min intervals. Plasmids used were pSB3004 (□, ■; 4 att sites), pSB3002 (□, ▲; 2 att sites) and pSB3000 (○, ●; no att sites). Panel B: luminescence (solid symbols, Relative Light Units; RLU) and absorbance (open symbols) were measured at 10 minute intervals. Plasmids used were pSB3014 (□, ■; 4 att sites), pSB3012 (□, ▲; 2 att sites) and pSB3010 (○, ●; no att sites). Data is presented as % maximal signal to allow direct comparison of repression kinetics despite the fact that light levels from each construct were different.