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Figure 6 | BMC Molecular Biology

Figure 6

From: Characterization of an Oct1 orthologue in the channel catfish, Ictalurus punctatus: A negative regulator of immunoglobulin gene transcription?

Figure 6

Endogenous Oct transcription factors bind the octamer motif. EMSA with nuclear extracts of the catfish B cell line 1G8. Lane 1, nuclear extract. Lane 2, nuclear extract + cold competitor. Lane 3, nuclear extract + scrambled competitor. Lane 4, nuclear extract + anti-Oct2 rabbit serum. Lane 5, nuclear extract + NRS (anti-Oct1 rabbit pre-bleed serum. Lane 6, nuclear extract + anti-Oct1 rabbit serum. Lane 7, nuclear extract + anti-Oct1 rabbit serum + anti-Oct2 rabbit serum. Oct1 and Oct2 shifts are designated by brackets. A lower bracket designates non specific binding labeled NS.

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BMC Molecular Biology

ISSN: 1471-2199

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