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Figure 3 | BMC Molecular Biology

Figure 3

From: Characterization of an Oct1 orthologue in the channel catfish, Ictalurus punctatus: A negative regulator of immunoglobulin gene transcription?

Figure 3

Catfish Oct1 fails to drive transcription from an octamer-dependent reporter construct. A schematic representation of the reporter construct (pO3-Δ56-CAT [31]) containing an octamer trimer is shown in A. The octamer motifs are shown upstream of the TATA box (from the minimal c-fos promoter) and the CAT reporter gene is shown as an open box. Transcriptional activation (as fold increase) was measured following co-transfection into the catfish B cell line (1G8) of the reporter construct and an Oct expression construct. A. Transcriptional activation driven by catfish Oct2β (pRc/CMV/Oct2β) or catfish Oct1 (pRc/CMV/Oct1). B. Transcriptional activation driven by catfish Oct2β (pRc/CMV/Oct2β), catfish Oct1 (pRc/CMV/Oct1) and human BOB.1 (pRc/CMV/hBOB.1), or by a co-transfection of catfish Oct2β with human BOB.1, or catfish Oct1 with human BOB.1. C. Negative transcriptional regulation by catfish Oct1. One pM of catfish Oct2β expression construct was co-transfected with increasing amounts of catfish Oct1 (0.5, 1.0, 2.0 pM respectively) and the transcriptional activation of the reporter construct measured. Values for five replicate transfections in A and four replicate transfections in B and C are shown as mean ± SEM. Statistical significance was calculated by the Student T test assuming unequal variances. One asterisk indicates a p value of less than .05 and two asterisks indicates a p value of less than .01.

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