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Table 1 Oligonucleotides used in gel shift assays *

From: GATA elements control repression of cardiac troponin I promoter activity in skeletal muscle cells

cTnI GATA#

5' CGCCTGTTATCGCTTATCCTGGG 3'

GCGGACAATAG CGAATAG GACCC

cTnI mut GATA → CACA

5' CGCCTGTTG TG GCTTG TG CTGGG 3'

GCGGACAA C A C CGAA C A C GACCC

cTnI mut GATA → GCCA

5' CGCCTGTTGG CGCTTGG CCTGGG 3'

GCGGACAA CC G CGAA CC G GACCC

BNP GATA

5'GATCCCAGGAATGTGTCTGATA AATCAGAGATA ACCCA 3'

  1. * GATA sites are underlined. Mutated nucleotides are indicated in bold.
  2. # This sequence comprises the two GATA elements closely associated between -67 and -57 of the cTnI promoter
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BMC Molecular Biology

ISSN: 1471-2199

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