Table 1 Oligonucleotide sequences

GFP-N-start

5'-tatgcggccgcgtcgac catggtgagcaagggcgaggagct-3'

GFP-C-end

5'-tatgcggccgc agtcga c ttgtacagctcgtccatgcc-3'

HAP1-157-forward

5'-tatgtcgac agaacgggacctgaacacagc-3'

HAP1-238-forward

5'-tatgtcg ac acagagggatcaagaccagca-3'

HAP1-300-forward

5'-tatgtcgac gatgctcattctggaatgtgtg-3'

HAP1-380-forward

5'-tatgtcgac ctcctacatgcaggattatggg-3'

HAP1-473-forward

5'-tatgtcgac agatctcaagccacctgaagat-3'

HAP1-261-reverse

5'-tatgcggc cgc tgtctcagccttagggtgt-3'

HAP1-306-reverse

5'-tatgcggccg c acacattccagaatgagcatc-3'

HAP1-386-reverse

5'-tatgcggccg c ccataatcctgcatgtaggag-3'

HAP1-478-reverse

5'-tatgcggccgc tcttcaggtggcttgagatctt-3'

HAP1-582-reverse

5'-tatgcggccgc gttggggagtcttttgggacca-3'

TBP-N-start-Sal I

5'-atcgtcgac tatggaccagaacaacagccttcca-3'

TBP-N-start-Bgl II

5'-tatagatct atggaccagaacaacagccttc-3'

TBP-N-end

5'-atagcggccgc ttaagagctctcagaagctggtgtggca-3'

TBP-C-start

5'-tatggatccaccatgg accagagctctggaattgtaccgcagcttca-3'

TBP-C-end

5'-gctagcggccgc ccaagtagcagcacagagc-3'

TBP-Q₅₀

5'-atactgcag ttgctactgc ctgttgctgctgttgctgctgctgttgctgttgctgctgttgctgttgctgctgttgttgctgctgctgttgctgctgctgctgttgttgctgctgttgctgctgttgctgctgctgttgttgctgctgctgttgctgttgctgctgctgtctttgttgctcttccaaaatagagagact-3'

TBP-ΔQ

5'-atgcactgcagtcgcga cagctccgccctcttccaaaatagagagact-3'

U2AF-N-start

5'-tatgtcgac catgtcggacttcgacgagttcgag-3'

U2AF-C-end

5'-tatgcggccgc cggtggtaagaatcagggtca-3'

linker (top strand)

5'-aattcgcggccgcgtcgac-3'

linker (bottom strand)

5'-phos-gtcgacgcggccgc g-3'