Figure 8From: HAP1 can sequester a subset of TBP in cytoplasmic inclusions via specific interaction with the conserved TBPCOREPolyQ dependence of relative nuclear and cytoplasmic TBP protein levels. (A) representative fluorescent photomicrographs used for quantitative analyses. Cells were transfected with CMVp-GFP-HAP1-A7–598 and either CMVp-DSRed2-TBP-FL, CMVp-DSRed2-TBP-ΔQ, or CMVp-DSRed2-TBP-Q50, as indicated at left. White dotted line delineates nuclei in each frame. (B) nuclear-cytoplasmic TBP distributions. Bars show average plus one standard deviation. Aserisks, cytoplasmic levels of TBP-ΔQ were significantly less than those of TBP-FL, P < 0.05. Cytoplasmic levels of TBP-FL and TBP-Q50 were not significantly different.Back to article page