Deletion analysis of the TBP interacting regions on HAP1. (A) The full-length HAP1-B protein is represented at top, including the location of the known acidic domain (AcD) and the two coiled coil domains. HAP1 amino acids encoded by each cDNA clone in pPC86 are shown at left. The presence (+) or absence (-) of interaction with TBP-C is shown at right. Results suggested that the TBP interacting regions on HAP1 were between amino acids 157 and 261, and between amino acids 473 and 582. (B) Amino acid sequences of HAP1 TBP-interacting regions.