Figure 6

Replication origin activity of the promoter region of the chicken adenosine receptor 2B gene. A. Schematic illustration of the chicken A2B receptor gene. The black boxes show the two exons of the gene. The transcriptional start site is marked by an arrow. The position of primer pairs used in the PCR analysis is shown below. The scale at the top is in kilobases. B. Nascent strand abundance assay of the adenosine receptor 2B promoter region using the PCR primer pairs indicated at the bottom. The columns show the average abundance of the corresponding sequences in nascent strand preparations of BM2 (black columns) or HD11 (white columns) cells relative to the sequences amplified with the -3.9 kb primer pair. Error bars mark the standard deviations. C. Nascent strand abundance analysis of the promoter region using wild-type (black columns) or c-myb deficient (white columns) DT40 cells.