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Figure 2 | BMC Molecular Biology

Figure 2

From: Site-specific acetylation of ISWI by GCN5

Figure 2

In vitro , ISWI is specifically acetylated by HATs able to modify histone H3. (A) The histone acetyltransferase activities of recombinant GCN5, p300 and MOF were assayed in reactions containing all four histones. Incorporation of [3H]-acetyl-coenzyme A leads to labelled proteins, which are separated by SDS-PAGE and detected by autoradiography of the gel. Bands corresponding to acetylated histones are marked by arrows. (B) Recombinant ISWI was incubated in acetylation reactions with GCN5, p300 or MOF. Acetylated ISWI (arrow) was detected as in (A). All other bands (asterisks) are due to auto-acetylation of the corresponding enzyme.

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