Figure 7
Effects of DM3 on TGFβ-induced growth inhibition and cell apoptosis. Panel A: Cell growth. The MCF-7 transfectants were seeded at a density of 5000 cells per well in 24-well plates followed by TGFβ1 treatment or not for 96 h. The cell counts for each transfectants were quantified by a standard MTT assay. The results are presented as the means ± SEM of three independent experiments. ** Indicates that the DM3 variant rescued the cells from TGFβ-induced growth suppression (P < 0.01). Panel B: TGFβ-induced cell apoptosis. Transfectants (2 × 105 cells) expressing DM3, DM4, or vector control (VC) were seeded and stimulated with (+) or without (-) 5 ng/mL of TGFβ1 for 72 h. The cells were collected and stained with Annexin V and propidium iodide for detection of apoptotic cells. The percentage of the apoptotic cells in the total cell population was determined by flow cytometry. Data are presented as the fold increases in cell apoptosis compared with those of corresponding untreated cells.