Inactivation of NMD increases viability of sup45 nonsense mutants in Saccharomyces cerevisiae

BMC Molecular Biology

Table 1 Nonsense mutations sequenced in the present work

Mutant allele	Nucleotide substitution		Putative destabilizing motif^a
	Position/ORF size	Codon change^b
his7-1	229/1659	A AA→T AA	³¹¹ca TTGAT t TT a agttgtccaggt TCGATG a TTC a
lys9-A21	605/1341	TT A→TA A	ND
trp1-289	403/678	C AG→T AG	⁴³⁶gtTTGAT t C agaagcaggtgggacaggtgaactT t T g GAT tggaacTCGAT t TCT gactgggttggaagg

^a The downstream sequences were searched for the presence of DSE (TGYYGATGYYYYY) [15]. The numbers above the sequences indicate the 5' boundaries of putative DSE (underlined sequences) in the corresponding open reading frame (ORF); ND – not detected. ^b Codon changes in the case of each mutation are shown, nucleotide changes are underlined.

ISSN: 1471-2199