Table 3 TaqMan primers and probes used in this study
Name | Target | Sequence (5'-3') |
|---|---|---|
Forward primers | Â | Â |
   TQEx2Fw | exon 2 | 5'-GCTCACCAACAGATGAAGAC |
   TQEx2cFw | exon 2c | 5'-CCTCCTTGGAGACCAGCTA |
   TQEx8Fw | exon 8 | 5'-GACCTGGCCCTATTCATTG |
Reverse primers | Â | Â |
   TQEx2aRev | exon 2a | 5'-GCATTGTTCAGACTCTTGGT |
   TQEx2eRev | exon 2e | 5'-CAGGGAAGGTTCAAGGTCAA |
   TQEx3Rev | exon 3 | 5'-GTATCTGACCCTGCTTTCCA |
   TQEx4Rev | exon 4 | 5'-TCTGAACGCAGATGGACCT |
   TQEx9Rev | exon 9 | 5'-ACCCGTGGAACGTTCATGA |
Probes | Â | Â |
   Ex2:2aPr | Exons 2:2a | 5'-FAM-ACCTCCTACCCCTCGTTGGTG-TAMRA |
   Ex2:2ePr | Exons 2:2e | 5'-FAM-CACCAACGAGATTATCTTGAAGAC-TAMRA |
   Ex2c:3Pr | Exons 2c:3 | 5'-FAM-CATTCCAGACCCTCACATAAGGA-TAMRA |
   Ex2:4Pr | Exons 2:4 | 5'-FAM-TCCCATCAGCCTCGTTGGTGC-TAMRA |
   Ex8:9Pr | Exons 8:9 | 5'-FAM-CTGGCCGGTCTCCACACAGAA-TAMRA |