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Table 3 TaqMan primers and probes used in this study

From: Alternative splicing of human peroxisome proliferator-activated receptor delta (PPARdelta):effects on translation efficiency and trans-activation ability

Name Target Sequence (5'-3')
Forward primers   
   TQEx2Fw exon 2 5'-GCTCACCAACAGATGAAGAC
   TQEx2cFw exon 2c 5'-CCTCCTTGGAGACCAGCTA
   TQEx8Fw exon 8 5'-GACCTGGCCCTATTCATTG
Reverse primers   
   TQEx2aRev exon 2a 5'-GCATTGTTCAGACTCTTGGT
   TQEx2eRev exon 2e 5'-CAGGGAAGGTTCAAGGTCAA
   TQEx3Rev exon 3 5'-GTATCTGACCCTGCTTTCCA
   TQEx4Rev exon 4 5'-TCTGAACGCAGATGGACCT
   TQEx9Rev exon 9 5'-ACCCGTGGAACGTTCATGA
Probes   
   Ex2:2aPr Exons 2:2a 5'-FAM-ACCTCCTACCCCTCGTTGGTG-TAMRA
   Ex2:2ePr Exons 2:2e 5'-FAM-CACCAACGAGATTATCTTGAAGAC-TAMRA
   Ex2c:3Pr Exons 2c:3 5'-FAM-CATTCCAGACCCTCACATAAGGA-TAMRA
   Ex2:4Pr Exons 2:4 5'-FAM-TCCCATCAGCCTCGTTGGTGC-TAMRA
   Ex8:9Pr Exons 8:9 5'-FAM-CTGGCCGGTCTCCACACAGAA-TAMRA
  1. The primers and probes are annotated according to the target exons and exon/exon junctions in the human PPARδ gene, respectively.