Figure 7

EMSA to assess the binding properties of PPARδ isoform 1 and 2 to ACO-PPRE. Gel retardation assay was performed with end-labelled ACO-PPRE in the presence (+) or absence (-) of in vitro transcribed/translated human PPARδ1, PPARδ2, RXRα or mock lysate as described in "Methods". Unlabelled ACO-PPRE was added at 100-fold molar excess for competition. Supershift experiments were carried out using a PPARδ antibody directed against the N-terminal region of human PPARδ. Arrows indicate the positions of the shifted and the supershifted bands. None of the receptors (PPARδ1, PPARδ2 or RXRα) alone could be supershifted in the presence of the PPARδ antibody (data not shown).