Figure 3

Reporter gene constructs and relative promoter activities. A schematic view of the different PPARδ reporter gene constructs and the luciferase activities obtained after transient transfection in Huh7 (black bars) and HeLa cells (white bars), setting the value of the promoterless control plasmid to 1. A. Luciferase assays on reporter gene constructs containing from 2.6 kb down to 48 bp of the region upstream of exon 1 in the PPARδ gene compared to empty pGL3 vector. B. Luciferase assay on reporter gene constructs containing approximately 1 kb and 250 bp of the upstream regions of four identified alternative 5'-ends summarized in Table 2 and illustrated in Figure 1B (5'-UTRs: H-K), compared to empty pGL3 vector.