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Figure 4 | BMC Molecular Biology

Figure 4

From: Reduction of gene repair by selenomethionine with the use of single-stranded oligonucleotides

Figure 4

(A) . Suppression of gene repair by p53 overexpression is equivalent to selenomethionine treatment. Cells were incubated with or without 200 μM selenomethionine for 24 hrs. 5 μg of respective overexpression plasmid (empty plasmid (pcDNA), wild-type p53 (P72), or mutant p53 (R175H)) and 8 μM EGFP3S/47NT oligonucleotide was electroporated in HCT 116 cells; gene repair levels were evaluated by FACS after 24 hours. ( B) . Selenomethionine treatment enhances gene repair in DLD-1 (p53+/- mutant). DLD-1 cells were treated with selenomethionine (50 μM, 100 μM, 200 μM) for 24 hours prior to electroporation of the oligonucleotide. The cells were analyzed by FACS 24 hours after electroporation. The data represents three (± S.D.) independent experiments.

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