Figure 2

(A) . Selenomethionine treatment suppresses gene repair. HCT 116 cells were treated with selenomethionine (50 μM, 100 μM, 200 μM) for 24 hours prior to electroporation of the oligonucleotide. The cells were analyzed by FACS 24 hours after electroporation. The data represents three (± S.D.) independent experiments. ( B) . Selenomethionine induces mild, reversible G2/M delay. HCT 116 cells were not treated or treated with selenomethionine (50 μM, 200 μM) for 24 hours and processed for analysis of cell cycle. The profiles were generated using ModFit LT software. For cells treated with selenomethionine for 24 hrs: no treatment (57.6 +/- 1.4%G1, 26.1 +/- 0.6%S,16.37 +/- 0.8% G2), 50 μM selenomethionine (50.7 +/- 1.6% G1,24.7 +/- 1.3%S,24.6 +/- 2.6% G2) 200 μM selenomethionine (43.8 +/- 2.27% G1,20.1 +/- 3.9% S,36.1 +/- 6.2 % G2). For cells treated with selenomethionine for 24 hrs, washed, and allowed to recover for 24 hrs: no treatment (55.2 +/- 3.3% G1,28.8+/- 1.8S,16.1 +/- 1.4%G2), 50 μM selenomethionine (50.8 +/- 2.9% G1, 29.8+/- 3.3% S,19.4+/- 0.4%G2) 200 μM selenomethionine (53.2 +/- 1.2% G1, 31.7+/- 1.9% S,15.1+/- 0.7 G2).