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Table 3 Oligonucleotide sequences used to prepare DNA substrates for the enzyme assays performed in this study

From: Enzymes involved in DNA ligation and end-healing in the radioresistant bacterium Deinococcus radiodurans

Oligonucleotide

Length (nt)

Sequence (5'-3')

RNA-5' nick

19

CAGCAGCAAAUGAAAAAUC

DNA-5' nick

19

CAGCAGCAAATGAAAAATC

RNA-3' nick

25

CCUGCAACAGUGCCACGCUGAGAGC

DNA-3' nick

25

CCTGCAACAGTGCCACGCTGAGAGC

DNA-3'P nick

25

CCTGCAACAGTGCCACGCTGAGAGC-P

DNA-opposite

46

AGATTTTTCATTTGCTGCTGGCTCTCAGCGTGGCACTGTTGCAGGC

Kinase-DNA

25

GCTTTCCGAGTACCGGGGTCTTCCG

  1. All oligonucleotides were PAGE purified and labelled as described in the Methods section. P stands for a 3' phosphate group.
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BMC Molecular Biology

ISSN: 1471-2199

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