Enzymes involved in DNA ligation and end-healing in the radioresistant bacterium Deinococcus radiodurans

BMC Molecular Biology

Table 2 K_M values of prokaryotic NAD⁺-dependent DNA ligases

Organism	T [°C]	K_M[nM]	Reference
D. radiodurans	30	105 ± 16	This work
E. coli	18	179	Georlette et al., 2000
E. coli	30	702	Georlette et al., 2000
E. coli	45	2040	Georlette et al., 2000
P. haloplanktis	4	165	Georlette et al., 2000
P. haloplanktis	18	296	Georlette et al., 2000
P. haloplanktis	25	631	Georlette et al., 2000
T. scotoductus	45	236	Georlette et al., 2000
T. scotoductus	60	465	Georlette et al., 2000

K_M values for nicked DNA substrates. Details for K_M determination of D. radiodurans NAD⁺-dependent DNA ligase are described in Methods. K_M is the mean of 3 independent experiments and the error is given as standard error of the mean.

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