Enzymes involved in DNA ligation and end-healing in the radioresistant bacterium Deinococcus radiodurans

BMC Molecular Biology

Table 1 PCR primer sequences used in this study

Primer name	Used for	Sequence (5'-3')
DRB0100F	cloning of DRB0100wt into pRSETb	CGCGGATCC GATGCGAGTCAAATACCCTTC
DRB0100R	cloning of DRB0100wt into pRSETb	CGCGGATCC GTCATGACTGCTCCTGGCG
DRB0100_mutF	introduction of K40A mutation into DRB0100	CGTCGTGACCGAGGC GCTCGACGGCG
DRB0100_mutR	introduction of K40A mutation into DRB0100	CGCCGTCGAGCGC CTCGGTCACGACG
DR2069F	cloning of DR2069 wt into pRSETb	CGCGGATCC GATGCGTTACCCTGGGCGC
DR2069R	cloning of DR2069 wt into pRSETb	CGCGGATCC GTCAGCTTTCAGCGGGGGC
mut_DR2069F	introduction of K128A mutation into DR2069	CCGGCGAGCTGGC AATCGACGGCCT
mut_DR2069R	introduction of K128A mutation into DR2069	CAGGCCGTCGATTGC CAGCTCGCCGG
DRB0098F	cloning of DRB0098 into pRSETb	CGCGGATCC GATGAACCGCAAAAACCGTAC
DRB0098R	cloning of DRB0098 into pRSETb	CGCGGATCC GTCAGGAGGTAGATGAGGGCAG
98_R371LF	introduction of R371K mutation into DRB0098	GGTCAGCTCGGAGCAAAA ATCAGCGGGAGAGAGC
98_R371LR	introduction of R371K mutation into DRB0098	GCTCTCTCCCGCTGATTTT TGCTCCGAGCTGACC

All oligonucleotides were desalted and used in a final concentration of 0.4 μM. T7 sequencing primers can be found at the Microsynth webpage. Bold bases represent those exchanged in the site-directed mutagenesis. Restriction sites are shown in italics.

ISSN: 1471-2199