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Table 1 Primers used in PCR and sequencing

From: Extraordinary diversity among members of the large gene family, 185/333, from the purple sea urchin, Strongylocentrotus purpuratus

Primer

Sequence (5' → 3')

Annealing site*

185-5'UTR

TAGCATCGGAGAGACCT

5'UTR

185-3'UTR

AAATTCTACACCTCGGCGAC

3'UTR

185-LR1

ATCRTYGCCATYSTGGCYG

Leader

185-F2

AAGMGATTWCAATGAACKRCGAG

Ex1/Er1

185-F5

GGAACYGARGAMGGATCTC

Ex25/Er27

185-F6

GAAGAAGAAACTGATGCTGCC

Ex7/Er6

185-R5

ACTCTGTACTGCGGAGAGCCGAC

Ex4/Er3, Ex5/Er4and Ex6/Er5

185-R6

GCAGCATCAGTTTCTTCKTCTC

Ex7/Er6

185-R9

CTTHARGTGGTGAARATGTCG

Ex25/Er27

185-R11

ATCTCCCAGGCGTGATG

Ex25/Er27

  1. *The annealing site indicates the elements in which the primers will hybridize in either the cDNA-based alignment (elements denoted as Ex; Figure 2) or repeat-based alignment (elements denoted as Er; Figure 6).
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BMC Molecular Biology

ISSN: 1471-2199

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