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Table 3 Primers, PCR conditions and qPCR efficiency

From: Selection of reference genes for gene expression studies in pig tissues using SYBR green qPCR

Gene symbol

Oligo sequence (5'→3')

Amplicon length

Tm (°C)

E%

ACTB

CACGCCATCCTGCGTCTGGA AGCACCGTGTTGGCGTAGAG

100

63

97

B2M1

CAAGATAGTTAAGTGGGATCGAGAC TGGTAACATCAATACGATTTCTGA

161

58

101

GAPDH

ACACTCACTCTTCTACCTTTG CAAATTCATTGTCGTACCAG

90

60

103

HMBS2

AGGATGGGCAACTCTACCTG GATGGTGGCCTGCATAGTCT

83

58

98

HPRT1

GGACTTGAATCATGTTTGTG CAGATGTTTCCAAACTCAAC

91

60

99

RPL4

CAAGAGTAACTACAACCTTC GAACTCTACGATGAATCTTC

122

60

97

SDHA

CTACAAGGGGCAGGTTCTGA AAGACAACGAGGTCCAGGAG

141

58

98

TBP1

AACAGTTCAGTAGTTATGAGCCAGA AGATGTTCTCAAACGCTTCG

153

60

93

YWHAZ

TGATGATAAGAAAGGGATTGTGG GTTCAGCAATGGCTTCATCA

203

60

99