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Table 2 RNA transcription levels (normalised Ct values) of the candidate reference genes. Values in parentheses shows the ranking of genes in the specific tissue calculated by GeNorm. Values in bold have an M-value above 1.5.

From: Selection of reference genes for gene expression studies in pig tissues using SYBR green qPCR

 

B2M

RPL4

ACTB

GAPDH

YWHAZ

SHDA

HPRT

TBP

HMBS

muscle

14.7(4)

16.6(7)

17.9(8)

13.2(5)

20.0(6)

19.5(9)

22.5(1)

23.8(1)

24.5(3)

adipose

15.8(-)

17.9(-)

16.5(-)

16.9(-)

17.4(-)

19.9(-)

20.8(-)

21.8(-)

22.8(-)

small intestine

12.8(3)

16.2(1)

16.5(8)

17.2(7)

19.4(5)

19.8(9)

22.7(4)

23.4(1)

22.8(6)

skin

15.4(5)

16.4(1)

16.7(6)

18.4(4)

18.3(1)

21.8(3)

23.3(9)

23.2(8)

24.6(7)

stomach

13.0(6)

17.3(3)

15.5(1)

18.9(7)

18.4(4)

21.1(9)

23.1(5)

23.7(8)

24.0(1)

pancreas

15.2(6)

16.2(8)

17.1(7)

20.6(1)

21.3(5)

21.2(9)

22.9(1)

23.7(3)

23.9(4)

kidney

12.9(5)

16.1(4)

15.8(1)

16.6(3)

18.2(7)

18.0(9)

21.2(1)

21.4(6)

22.4(8)

lung

12.5(6)

16.8(1)

15.4(3)

18.8(7)

18.6(1)

21.4(9)

21.2(8)

22.5(4)

24.0(5)

thymus

14.6(3)

16.3(1)

15.3(8)

17.4(4)

17.5(7)

21.6(9)

22.0(1)

21.8(5)

23.7(6)

hippocampus

13.2(7)

16.2(9)

15.8(5)

18.3(4)

18.1(3)

21.0(6)

22.1(8)

22.8(1)

24.2(1)

liver

13.2(9)

16.8(5)

16.5(4)

17.7(6)

21.0(7)

19.8(1)

19.8(3)

23.1(1)

23.3(8)

cortex cerebri

15.6(3)

17.1(4)

16.2(1)

16.3(1)

16.9(6)

19.2(8)

20.0(9)

21.7(5)

23.0(7)

bone marrow

15.1(3)

17.2(7)

16.4(8)

19.1(5)

19.8(4)

21.9(6)

21.6(1)

22.5(1)

19.9(9)

cerebellum

16.7(9)

17.2(7)

17.3(4)

17.6(8)

17.9(1)

20.9(6)

20.9(5)

21.8(1)

23.9(3)

lymph nodules

15.3(1)

16.3(5)

16.2(6)

19.2(9)

18.9(3)

20.3(8)

21.3(7)

20.2(4)

23.6(1)

heart

13.9(4)

15.8(1)

16.9(3)

14.6(8)

17.6(6)

17.1(7)

21.2(1)

21.5(5)

22.9(9)

bladder

14.9(-)

15.5(-)

16.3(-)

19.2(-)

18.7(-)

21. (-)

21.5(-)

21.5(-)

23.8(-)