Figure 5
Nucleolin depletion induces the formation of micronuclei and multinuclear cells. A. Images of HeLa cells or human primary fibroblasts transfected for 4 days with scrambled siRNA #1 or with nucleolin siRNA mix #2 and #4 were counterstained with DAPI and visualized at the same magnification. Micronuclei are observed on A6 and A7, multinuclear cells are observed on A3, A4 and A8, large nuclei are observed on A2 and A6. Bar represents 10 μm. B. Table shows the percentage of multinuclear cells, and cells with large nuclei or micronuclei in HeLa cells or primary human fibroblasts, determined by cell counting under the microscope. n = 1740 for HeLa cells transfected with scrambled siRNA #1, n = 1380 for HeLa cells transfected with nucleolin siRNA mix, n = 1560 for fibroblasts transfected with scrambled siRNA #1, n = 1220 for fibroblasts transfected with nucleolin siRNA mix.