Figure 6

Immunogold localisation of Ta ASY1 polyclonal antibody using anther sections from T. aestivum. (A) Immunogold labelling of meiotic cells at early zygotene showing localisation of Ta ASY1 to dense axial elements (bar = 0.5 μm) (B) Low magnification image of a meiotic cell at late zygotene to early pachytene, containing formed SCs, as illustrated by the SC within the boxed area, which is shown in (C). (C) Immunogold labelling displays localisation of Ta ASY1 to chromatin regions associated with the lateral elements. Chromosome axes (lateral elements) are indicated with arrows, and the central element is indicated with an arrow head. (D) Diplotene meiotic cell showing loss of Ta ASY1 localisation once the SC has disassociated and chromosomes have condensed further. (E) Negative control excluding the primary antibody. Arrows indicate chromosome axes and the arrowhead indicates the central element. All sections were counterstained with uranyl acetate and lead citrate.