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Figure 2 | BMC Molecular Biology

Figure 2

From: A flow cytometry technique to study intracellular signals NF-κB and STAT3 in peripheral blood mononuclear cells

Figure 2

Flow cytometry of simultaneous phosphorylated NF-κB and STAT3 expression from B-cells, T-lymphocytes and monocytes/macrophages stimulated by IL1β (control). Cytogram (A and C) depicted one experiment and showed the isotype control (A1 and C1 for B-cells, A4 and C4 for T-lymphocytes and A7 and C7 for monocytes/macrophages). Cytogram (A) showed the pNF-κB translocation of B-cells (A2, A3), T-lymphocytes (A5, A6) and monocytes/macrophages (A8, A9) with (A3, A6, A9) or without (A2, A5, A8) stimulus IL1β (50 ng/mL) for 30 min. Cytogram (C) showed the pSTAT3 translocation of B-cells (C2, C3), T-lymphocytes (C5, C6) and monocytes/macrophages (C8, C9) with (C3, C6, C9) or without (C2, C5, C8) stimulus IL1β (50 ng/mL) for 30 min. Data were representative of seven experiments. Summary of flow cytometry analysis (n = 7) of percentage of phosphorylated NF-κB (B) and phosphorylated STAT3 (D) activation (versus untreated) from B-cells, T-lymphocytes and monocytes/macrophages. The graphs represent the difference in percentage of phosphorylated nuclear factor between stimulated and untreated cells. Statistical significance (wilcoxon paired test; p < 0.05) was represented by an asterisk (*). Data represented the mean (± SD) of seven experiments.

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