Western blot and ELISA analysis of phosphorylated NF-κB and STAT3 in PBMCs. Western Blot of pNF-κB (A, C) and pSTAT3 (E) in PBMCs nuclear extract was observed with or without stimuli: IL1β (50 ng/mL) (A), sCD40L (50 ng/mL) (C) or IL10 (100 ng/mL) (E) for various periods (0 to 30 min). Data were representative of five experiments. pNF-κB and pSTAT3 were detected by ELISA (n = 5) in activated PBMCs nuclear extracts and the time dependant effect of the PBMCs stimulus was observed. PBMCs were stimulated for various periods of time (0 to 30 min) with or without IL1β (B), sCD40L (D) or IL10 (F). Statistical significance (wilcoxon paired test; p < 0.05) was represented by an asterisk (*). Data represented the mean (± SD) of five experiments.