Figure 5

siRNA directed against the HIV-1 TAR structure can potentially downregulate LTR-driven viral expression. 293 cells were transfected with 100 nanograms of pLTR-Luc, 100 nanograms of pRL-CMV and 25 nanograms of RNA and assayed 48 hours later for firefly and renilla luciferase expression. Cells were transfected with 25 nanograms siGFP, 25 nanograms siGL2, 5 and 25 nanograms of TAR-WT, TAR-D, TAR-A, siTAR1 or siTAR2. RNA levels were normalized to 25 nanograms using siGFP.