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Table 1 A list of the gene annotations, functions, primer and probe sequences, and qPCR metrics for the 12 new reference genes, and glyceraldehyde 3-phosphate dehydrogenase

From: Identification of new reference genes for the normalisation of canine osteoarthritic joint tissue transcripts from microarray data

Gene Name Gene Function Gene Symbol Accession Number [GenBank] Forward(F) and Reverse(R) Primers Probe Sequence Average Standard Deviation of Triplicate R2 PCR Efficiency
CG14980-PB Protein coding C7orf28B XM_536878 F-gcaggaagggattctccag R-ggtccagtaagaaatcttccataa gccaggaa 19.8 0.986 104.3
Glyceraldehyde-3-phosphate dehydrogenase Enzyme in the glycolysis/gluconeogenesis pathway GAPDH NM_001003142 F-ctggggctcacttgaaagg R-caaacatgggggcatcag ctgctcct 20.3 0.991 101.1
Gu binding protein Nuclear receptor in transcriptional co-regulation PIAS1 XM_535524 F-ggagacaatcagcattataacacct R-tgatcatctgacactgctgct ggctgctg 16.9 0.990 99.6
HIRA interacting protein 5 isoform 2 Histone-interaction-DNA packaging HIRP5 XM_850340 F-aattcagaacatgctgcaatttta R-tgattcatcatccataacctgttc aggtggag 8.6 0.998 96.9
Hematopoietic stem/progenitor cells 176 Transport protein particle involved in endoplasmic reticulum to Golgi vesicle transport TRAPPC2L XM_844929 F-gatgatccaggtgtgctgag R-caatacggttatgtcaacagcact ctggagga 25.2 0.993 97.2
5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase Purine biosynthesis ATIC XM_858011 F-cgctgcctctttcaaacat R-tttggcctcatcttcactgag cagcaggt 13.4 0.991 97.7
Mitogen-activated protein kinase 6 Phosphorylates microtubule-associated protein 2 (MAP2) MAPK6 XM_858091 F-tcttcttgggatagccagtttg R-cctcacctcacaacaaaactgat ggtggtgg 14.9 0.992 97.6
Mitochondrial 28S ribosomal protein S25 Mitochondrial ribosomal subunit protein synthesis MRPS25 XM_533729 F-tgaaggtcatgacggtgaac R-tggatctgaggtatgttgaaaaac gccaggaa 14.6 1.000 95.5
Cytoplasmic protein NCK2 Regulates cell proliferation NCK2 XM_538440 F-cagacgctctacccgttca R-gtctcgcccttctcgaagtt aggaggag 28.7 0.975 96.7
ORM1-like 2 Protein folding in the endoplasmic reticulum ORMDL2 XM_843143 F-atggactacgggctccaat R-ctggccaggaggtagagtaca ctcctccc 28.2 0.996 103.1
Phosphatidylserine synthase I Membrane bound protein that catalyses the replacement of phospholipids by L-serine PTDSS1 XM_849686 F-actcagaatgcgacgatgg R-tcagaaccttttgaacctttcg ctggtctc 15.3 0.996 100.9
Mitochondrial ribosomal protein S7 Mitochondrial protein synthesis MRPS7 XM_846915 F-agtgcagggagaagaagcac R-cagcagctcgtgtgacaact ggatgctg 12.1 0.998 100.8
Transketolase Enzyme in pentose phosphate pathway TKT XM_533792 F-caacttctgtggctcccact R-ccagatcttccagagccatc tggggaag 11.8 0.993 103.4