Table 1 A list of the gene annotations, functions, primer and probe sequences, and qPCR metrics for the 12 new reference genes, and glyceraldehyde 3-phosphate dehydrogenase
Gene Name | Gene Function | Gene Symbol | Accession Number [GenBank] | Forward(F) and Reverse(R) Primers | Probe Sequence | Average Standard Deviation of Triplicate | R2 | PCR Efficiency |
|---|---|---|---|---|---|---|---|---|
CG14980-PB | Protein coding | C7orf28B | XM_536878 | F-gcaggaagggattctccag R-ggtccagtaagaaatcttccataa | gccaggaa | 19.8 | 0.986 | 104.3 |
Glyceraldehyde-3-phosphate dehydrogenase | Enzyme in the glycolysis/gluconeogenesis pathway | GAPDH | NM_001003142 | F-ctggggctcacttgaaagg R-caaacatgggggcatcag | ctgctcct | 20.3 | 0.991 | 101.1 |
Gu binding protein | Nuclear receptor in transcriptional co-regulation | PIAS1 | XM_535524 | F-ggagacaatcagcattataacacct R-tgatcatctgacactgctgct | ggctgctg | 16.9 | 0.990 | 99.6 |
HIRA interacting protein 5 isoform 2 | Histone-interaction-DNA packaging | HIRP5 | XM_850340 | F-aattcagaacatgctgcaatttta R-tgattcatcatccataacctgttc | aggtggag | 8.6 | 0.998 | 96.9 |
Hematopoietic stem/progenitor cells 176 | Transport protein particle involved in endoplasmic reticulum to Golgi vesicle transport | TRAPPC2L | XM_844929 | F-gatgatccaggtgtgctgag R-caatacggttatgtcaacagcact | ctggagga | 25.2 | 0.993 | 97.2 |
5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase | Purine biosynthesis | ATIC | XM_858011 | F-cgctgcctctttcaaacat R-tttggcctcatcttcactgag | cagcaggt | 13.4 | 0.991 | 97.7 |
Mitogen-activated protein kinase 6 | Phosphorylates microtubule-associated protein 2 (MAP2) | MAPK6 | XM_858091 | F-tcttcttgggatagccagtttg R-cctcacctcacaacaaaactgat | ggtggtgg | 14.9 | 0.992 | 97.6 |
Mitochondrial 28S ribosomal protein S25 | Mitochondrial ribosomal subunit protein synthesis | MRPS25 | XM_533729 | F-tgaaggtcatgacggtgaac R-tggatctgaggtatgttgaaaaac | gccaggaa | 14.6 | 1.000 | 95.5 |
Cytoplasmic protein NCK2 | Regulates cell proliferation | NCK2 | XM_538440 | F-cagacgctctacccgttca R-gtctcgcccttctcgaagtt | aggaggag | 28.7 | 0.975 | 96.7 |
ORM1-like 2 | Protein folding in the endoplasmic reticulum | ORMDL2 | XM_843143 | F-atggactacgggctccaat R-ctggccaggaggtagagtaca | ctcctccc | 28.2 | 0.996 | 103.1 |
Phosphatidylserine synthase I | Membrane bound protein that catalyses the replacement of phospholipids by L-serine | PTDSS1 | XM_849686 | F-actcagaatgcgacgatgg R-tcagaaccttttgaacctttcg | ctggtctc | 15.3 | 0.996 | 100.9 |
Mitochondrial ribosomal protein S7 | Mitochondrial protein synthesis | MRPS7 | XM_846915 | F-agtgcagggagaagaagcac R-cagcagctcgtgtgacaact | ggatgctg | 12.1 | 0.998 | 100.8 |
Transketolase | Enzyme in pentose phosphate pathway | TKT | XM_533792 | F-caacttctgtggctcccact R-ccagatcttccagagccatc | tggggaag | 11.8 | 0.993 | 103.4 |