Figure 3
From: An improved genetic system for detection and analysis of protein nuclear import signals
Western blot analysis of the expression of the various chimeras. Extracts from L40 strain yeast transformed with the indicated expression constructs were analyzed by Western blot to ensure expression of the HA tagged fusions. All samples were prepared and analyzed concurrently. 30 μg of extract was loaded per lane. Molecular weight markers are indicated on the left. Bands of interest are indicated with arrows. Top – pNIA-2μ, Middle – pNIA-CEN, Bottom – pNIA-CEN-MBP. Tag NLS – SV40 large T antigen NLS