Figure 8
Effect of nucleotide addition and deletion at the lower part of the stem-loop structure of the 83-nt RNA template on RNA synthesis initiation. (A) Sequences and secondary structures of wild-type 83-nt RNA and its derivatives. Names and ρ G are shown below the structures predicted by the Mfold program. Partial structures representing the lower stem-region are shown. The deleted and added nucleotides are indicated by ρ and boxed, respectively. (B) RNA synthesis directed by the wild-type 83-nt RNA and its derivatives. RdRp assays were performed with the RNA templates indicated above the autoradiogram, and products were resolved a 5% polyacrylamide sequencing gel (20 × 40 cm) containing 8 M urea. Arrowhead indicates the 81-nt internally initiated RNA product. Representative data from three independent experiments are shown.